| 1. | Establishment of an engineered cho - hm1r cells and the effect of acetylcholine on intracellular calcium concentration in the engineered cells
三氧化二砷对小鼠膀胱癌细胞系细胞内游离钙浓度影响的实验研究 |
| 2. | Furthermore , the therapy could be modified to improve the expression and function of the new t - cell genes , as well as making sure that more of the engineered cells survive
而且，这种治疗能通过提升t细胞基因的表现和功能得到改良，同时确保更多的基因工程细胞存活。 |
| 3. | Trials are also under way on the effectiveness of using radiation therapy to deplete a patient ' s supply of unmodified t - cells before replacing them with engineered cells
实验也发挥了放疗的效力，运用放疗先将病人体内未修改的t细胞供应耗尽，然后用基因工程细胞替代。 |
| 4. | However , this approach ( generically termed " cell therapy " and including stem cell therapy ) still has limitations : the cells that the engineered cells are designed to replace must be eliminated
然而，这种方法（一般称为"细胞疗法" ，包括干细胞疗法）仍然有局限性：工程细胞被设计去置换的那些细胞必须被除去。 |
| 5. | Thus , strenghtening the basic biology theorical study of the engineering cell and seeking the better cell system for the baculovirus of silkworm are current needs for the study of silkworm expression system
因此，加强对工程细胞的生物学基础理论研究，寻找更适宜于家蚕杆状病毒表达系统的家蚕细胞系是当前家蚕表达系统研究发展的需要。 |
| 6. | The isolated hepatocytes , isolated perfused organs , tissue slices , liver cell lines , subcellular fractions and the genetically engineered cells are the most commonly used in vitro liver models nowadays , especially the isolated hepatocytes
目前，常用的肝脏体外模型包括原代肝细模型、离体肝脏模型、肝脏切片模型、肝细胞系模型、亚细模型及基因工程细模型等，其中原代肝细型最?常用。 |
| 7. | It was applied widely to various fields of biology , medicine and agriculture . as engineering cells in the insect baculovirus expression system , insect cells have close relationship with to transform and expression efficiency in the expression system
昆虫细胞作为昆虫杆状病毒表达系统中的工程细胞，与表达系统的转化和表达效率密切相关，因此，昆虫细胞的研究对于表达系统的开发具有深远的影响作用。 |
| 8. | By the technology of gene cloning , bioconversion of d - amino acids with engineered cells containing d - hydantoinase and d - carbamoylase would be expected to overcome the drawbacks presented by using the original strains described above . according to the reported amino acid sequence of d - hydantoinases , two primers were designed and synthesized
本文根据文献报道的海因酶基因序列及大肠杆菌对密码子的偏爱性分别设计了正向及反向引物，以基因组dna为模板，利用pcr技术扩增得到菌株pseudomonasputidayz - 26和sinorhizobiummorelensess - ori的d -海因酶基因。 |
| 9. | This dissertaion , on the basis of the other studies , on the one hand , researched and analysed the chromosomes and mitoses of the cell line - bmn , which is widely used in the silkworm baculovirus expression system as an engineering cell . on the other hand , the dissertation attempted to explore establishment of the new silkworm embryoni cell line by primary culture
同时，家蚕体外细胞培养研究的进行，不仅可以为家蚕细胞生物学的基础理论研究提供良好的研究系统，而且在家蚕资源的开发与利用方面也具有重大的意义。本研究在借鉴前人研究的基础上，一方面对现在广泛应用于家蚕杆状病毒表达系统的工程细胞? bmn细胞的染色体和有丝分裂进行了研究与分析。 |
